Recent Activities

Scientific paper 

Metazoan diversity and its drivers: an eDNA survey in the Pacific Gateway of a changing Arctic Ocean
2025 Environmental DNA. DOI:10.1002/edn3.70082
Gerlien Verhaegen, Tatsuya Kawakami, Ayla Murray, Akihide Kasai and Charlotte Havermans

Long-term increasing trends of water quality in Hakodate Bay, Hokkaido, driven by meteorological factors
2025 Regional Studies in Marine Science. DOI: 10.1016/j.rsma.2025.104123
Chalvin Putra Rura and Akihide Kasai

Overwintering fish community in ice-covered Saroma-ko Lagoon, Hokkaido, Japan, inferred from metabarcoding-based detection of fish environmental DNA. 

2025 Environmental DNA. DOI: 10.1002/edn3.70068. 

Tatsuya Kawakami, Makoto Ozaki, Aya Yamazaki, Daiki Nomura and Akihide Kasai  

  

Seasonal changes in the protist communities of Hakodate Bay, southern Hokkaido, from 2020 to 2022. 

2024 Regional Studies in Marine Science, 78, DOI: 10.1016/j.rsma.2024.103775. 

Yusuke Hamao, Kyosei Morimoto, Shoko Tatamisashi, Masahide Wakita, Akihide Kasai, and Kohei Matsuno 


Comparison of fish species richness and community between the two river estuaries in Hakodate Bay based on a one-year survey. 

2024 Coastal Marine Science, 47, 8-37. 

Hyojin Ahn, Edouard Lavergne, Yoh Yamashita, Akihide Kasai 

 

Multidisciplinary research for sea ice in Saroma-ko Lagoon, Hokkaido, Japan 2023. 

2024 Bulletin of Glaciological Research, 42, 19-37. doi: 10.5331/bgr.24R01 

Daiki NOMURA, Ryota AKINO, Matthew CORKILL, Keizo HIRANO, Akihide KASAI, Seiji KATAKURA, Yusuke KAWAGUCHI, Tatsuya KAWAKAMI, Riri KIMURA, Delphine LANNUZEL, Ryosuke MAKABE, Mirai MATSUURA, Kohei MATSUNO, Klaus MEINERS, Keizo NAGASAKI, Yuichi NOSAKA, Nana SAMORI, Shinnosuke SAKAYA, Eun Yae SON, Ryotaro SUGA, Yumi SUNAKAWA, Keigo D. TAKAHASHI, Masaharu TAKAHASHI, Yuka TAKEDA, Takenobu TOYOTA, Manami TOZAWA, Pat WONGPAN, Hiroshi YOSHIDA, Kazuhiro YOSHIDA, Masaki YOSHIMURA 

 

Linkage between spatiotemporal distribution of environmental DNA and phenological activity in an amphidromous fish, ayu Plecoglossus altivelis altivelis, in a river located in its northernmost distributional area. 

2024 Journal of Fish Biology. DOI: 10.1111/jfb.15687. 

Takuma Yonago, Tatsuya Kawakami and Akihide Kasai 

 

Distribution and habitat preference of polar cod (Boreogadus saida) in the Bering and Chukchi Seas inferred from species-specific detection of environmental DNA. 

2023. Frontiers in Marine Science, DOI: 10.3389/fmars.2023.1193083. 

Kawakami T, Yamazaki A, Jiang H, Ueno H and Kasai A


Evaluation of community science monitoring with environmental DNA for marine fish species: “Fish survey project using environmental DNA”
2023 Environmental DNA  http://doi.org/10.1002/edn3.425
Suzuki-Ohno Y, Tanabe A, Kasai A, Masuda R, Seino S, Dazai A, Suzuki S, Abe T and Kondoh M


Evaluating the effects of filtration volume and filter pore size on detection of fish environmental DNA using metabarcoding for practical use in the open ocean
2023 Ecology and Evolution https://doi.org/10.1002/ece3.9921
Kawakami T
, Yamazaki A, Asami M, Gotoh Y, Yamanaka H, Hyodo S, Ueno H, and Kasai A

Inshore migration of Japanese eel Anguilla japonica encouraged by active horizontal swimming during the glass eel stage 2023 Fisheries Oceanography,  https://doi.org/10.1111/fog.12637 Karaki T, Sakamoto K, Yamanaka G, Kimura S, and Kasai A 


Otolith isotopic characterization as a nursery habitat indicator for stone flounder Platichthys bicoloratus
2022 Estuarine Coastal and Shelf Sciences, DOI: 10.1016/j.ecss.2022.108028
Amano Y, Otake T, Togashi H, Wada T, Kasai A, Kato Y, Yoshizumi C, Tayasu I, Kurita Y, and Shirai K


Atmosphere–sea ice–ocean interaction study in Saroma-ko Lagoon, Hokkaido, Japan 2021
2022. Bulletin of Glaciological Research, 40, 1-17. DOI: 10.5331/bgr.21R02
Nomura D, Ikawa H, Kawaguchi Y, Kanna N, Kawakami T, Nosaka Y, Umezawa S, Tozawa M, Horikawa T, Sahashi R, Noshiro T, Kaba I, Ozaki M, Kondo F, Ono K, YABE IS, Yae Son E, Toyoda T, Kameyama S, Wang C, Obata H, Ooki A, Ueno H, and Kasai A

Watershed proportion of forest cover fostering the conservation of red-listed fish richness in estuaries

2021. Conservation Biology.

Lavergne E, Kume M, Ahn H, Henmi Y, Terashima Y, Ye F, Kameyama S, Kai Y, Kadowaki K, Yamashita Y, and Kasai A 


Intraspecific genetic variability and diurnal activity affect environmental DNA detection in Japanese eel
2021. PLOSONE, DOI:10.1371/journal.pone.0231718
Takahashi S, Takada S, Yamanaka H, Masuda R and Kasai A

Distribution of Japanese eel Anguilla japonica revealed by environmental DNA
2021. Frontiers Ecology and Revolution, DOI:10.3389/fevo.2021.621461  

Kasai A, Yamazaki A, Ahn H, Yamanaka H, Kameyama S, Masuda R, Azuma N, Kimura S, Karaki T, Kurokawa Y and Yamashita Y

Factors structuring estuarine and coastal fish communities across Japan using environmental DNA metabarcoding

2021. Ecological Indicators, DOI: 10.1016/j.ecolind.2020.107216

Kume M, Lavergne E, Ahn H, Terashima Y, Kadowaki K, Ye F, Kameyama S, Kai Y, Henmi Y, Yamashita Y and Kasai A

An illustrated manual for environmental DNA research: Water sampling guidelines and experimental protocols

2020. Environmental DNA, DOI: 10.1002/edn3.121

Minamoto T, Miya M, Sado T, Seino S, Doi H, Kondoh M, Nakamura K, Takahara T, Yamamoto S, Yamanaka H, Araki H, Iwasaki W, Kasai A, Masuda R and Uchii K

Evaluation of biodiversity of five estuaries in Japan using environmental DNA metabarcoding method

2020. PLOSONE, DOI: 10.1371/journal.pone.0231127

Ahn H, Kume M, Terashima Y, Ye F, Kameyama S, Miya M, Yamashita Y and Kasai A


Traditional land use effects on nutrient export from watersheds to coastal seas

2020. Nutrient Cycling in Agroecosystems, DOI: 10.1007/s10705-020-10102-9

Sugimoto R, Kasai A, Tait DR, Rihei T, Hirai T, Asai K, Tamura Y and Yamashita Y


The effect of temperature on environmental DNA degradation of Japanese eel 

2020. Fisheries Science, 86, 465-471. DOI: 10.1007/s12562-020-01409-1

Kasai A, Takada S, Yamazaki A, Masuda R and Yamanaka H


Quantitative estimation of the ecosystem services supporting the growth of Japanese chum salmon

2020. Deep Sea Research Part II,  DOI: 10.1016/j.dsr2.2019.104702

Karasawa Y, Ueno H, Tanisugi R, Dobashi R, Yoon S, Kasai A and Kiyota M 

Winter monsoon promotes the transport of Japanese temperate bass Lateolabrax japonicus eggs and larvae toward the innermost part of Tango Bay, the Sea of Japan

2020. Fisheries Oceanography, 29, 66-83. DOI: 10.1111/fog.12450

Suzuki K, Fuji T, Kasai A, Itoh S, Kimura S and Yamashita Y 

Correlation of changes in seasonal distribution and catch of red sea bream Pagrus major with winter temperature in the eastern Seto Inland Sea, Japan (1972-2010)

2020. Fisheries Oceanography, 29, 1-9. DOI: 10.1111/fog.12432

Yamamoto M, Omi H, Yasue N and Kasai A

Dispersion and degradation of environmental DNA from caged fish in a marine environment

2019. Fisheries Science, 85, 327-337. DOI: 10.1007/s12562-018-1282-6

Murakami H, Yoon S, Kasai A, Minamoto T, Yamamoto S, Sakata MK, Horiuchi T, Sawada H, Kondoh M, Yamashita Y and Masuda R 


Age determination and growth pattern of temperate seabass Lateolabrax japonicus in Tango Bay and Sendai Bay, Japan

2019. Fisheries Science, 85, 81-98.DOI: 10.1007/s12562-018-1271-9

Jiang W, Lavergne E, Kurita Y, Todate K, Kasai A, Fuji T and Yamashita Y

Modeling the coastal ecosystem complex: present state and challenges

2018. Fisheries Science, 84, 293-307. DOI: 10.1007/s12562-018-1181-x

Itoh S, Takeshige A, Kasai A and Kimura S

Upstream migration mechanisms of juvenile temperate seabass Lateolabrax japonicus in the stratified Yura River estuary

2018. Fisheries Science, 84, 163-172. DOI: 10.1007/s12562-017-1167-0

Fuji T, Kasai A and Yamashita Y


Partial migration of juvenile temperate seabass Lateolabrax japonicus: a versatile survival strategy

2018. Fisheries Science, 84, 153-162. DOI: 10.1007/s12562-017-1166-1

Kasai A, Fuji T, Suzuki KW and Yamashita Y


大分県国東半島宇佐地域におけるウナギ石倉漁とその生態学的役割

2018. 水産学会誌, 84(1), 45-53. jstage

原田真実・久米学・望岡典隆・田村勇司・神崎東子・橋口峻也・笠井亮秀・山下洋


Food composition for blue mussels (Mytilus edulis) in the Menai Strait, UK, based on physical and biochemical analyses 

2017. Journal of Shellfish Research, 36, 659-668. DOI: 10.2983/035.036.0315

Morioka H, Kasai A, Miyake Y, Kitagawa T and Kimura S

Relative contributions of external forcing factors to circulation and hydrographic properties in a micro-tidal estuary

2017. Estuarine, Coastal and Shelf Science, 198, 225-235. DOI: 10.1016/j.ecss.2017.09.017

Yoon S and Kasai A

Estuarine circulation-driven entrainment of oceanic nutrients fuels coastal phytoplankton in an open coastal system in Japan

2017. Estuarine, Coastal and Shelf Science, 184, 126-137. DOI: 10.1016/j.ecss.2016.10.031

Watanabe K, Kasai A, Fukuzaki K, Ueno M and Yamashita Y 

Dynamics of iron in the Chikugo River Basin: Comparison of iron with nitrogen and phosphate input to the estuary

2016. Regional Studies in Marine Sciences, 8, 89-98. DOI: 10.1016/j.rsma.2016.09.007

Yamamoto M, Liu D, Kasai A, Okubo K and Tanaka M

Circulation and haline structure of a microtidal bay in the Sea of Japan influenced by the winter monsoon and the Tsushima Warm Current 

2016. Journal of Geophysical Research. DOI: 10.1002/2015JC011441.

Itoh S, Kasai A, Takeshige A, Zenimoto K, Kimura S, Suzuki K, Miyake Y, Funahashi T, Yamashita Y and Watabane Y

Relative contribution of estuarine nursery areas to the adult population of the temperate seabass Lateolabrax japonicus in the Tango Sea, as revealed by otolith Sr:Ca ratios

2016. Fisheries Oceanography, 25, 448-456. DOI: 10.1111/fog.12164

Fuji T, Kasai A, Ueno M and Yamashita Y

Isotopic trophic-step fractionation of the freshwater clam Corbicula sandai

2016. Fisheries Science, 82, 491-498. DOI: 10.1007/s12562-016-0970-3

Kasai A, Ishizaki D and Isoda T

Key biogeochemical processes evaluated by the stable nitrogen isotopes of dissolved inorganic nitrogen in the Yodo River estuary, Japan

2016. Biogeochemistry, 128, 1–17. DOI: 10.1007/s10533-016-0190-z

Sugimoto R and Kasai A

環境DNAを用いた沿岸域における魚類モニタリング

2016. 沿岸海洋研究,53, 173-178.DOI: 10.32142/engankaiyo.53.2_173

源利文・山本哲史・笠井亮秀・近藤倫生

The importance of estuarine production of large prey for the growth of juvenile temperate seabass (Lateolabrax japonicus)

2016. Estuaries and Coasts, 39, 1208-1220. DOI: 10.1007/s12237-015-0051-3

Fuji T, Kasai A, Ueno M and Yamashita Y

Book

豊かな内水面水産資源の復活のために-宍道湖からの提言,生物研究社(東京),山室真澄編,2020.

ヤマトシジミの好適餌料の推定および数値シミュレーションによるヤマトシジミ浮遊幼生の輸送・生残実験,45-51.

Connectivity of Hills, Humans and Oceans -Challenge to improvement of watershed and coastal environments. Kyoto University Press, Edited by N Shimizu, R Tateno, A Kasai, H Mukai and Y Yamashita.


Review

環境DNAを用いた生物多様性の評価と水産資源推定の可能性

2020 JATAFFジャーナル,8(2), 34-39.

笠井亮秀

資源環境・漁場環境

2018日本水産学会85周年記念誌,141-143.

笠井亮秀

流動モデルを用いた環境DNA濃度分布の再現

2018海洋と生物,234, 23-27.

笠井亮秀・尹錫鎭


Atlantisを用いた沿岸域生態系モデルの構築

2016 月刊海洋,48(7),315-320.

竹茂愛吾,伊藤幸彦,笠井亮秀,Yin Shengle,木村伸吾

沿岸域の物質循環と生物生産機構に関する研究

2016 水産海洋研究,80(2), 146-152.

笠井亮秀


Keynote speech

Environmental DNA metabarcoding as a tool of Biodiversity Assessment

International Biodiversity Conference 2019, November 11-15, 2019, Honey's Hotel, Mati, Philippines

Akihide Kasai 

環境DNA分析によるニホンウナギの分布推定

2019年度環境DNA学会第2回神戸大会,2019年11月4日,神戸大学鶴甲第2キャンパス 

笠井亮秀

環境DNA分析から見たニホンウナギの分布

うな丼の未来シンポジウム,2019年7月8日,東京大学農学部

笠井亮秀 

環境 DNA を用いた海洋生物モニタリングの現状と今後の展望

平成 29 年度東北ブロック底魚研究連絡会議,2018年2月27日,八戸商工会議所

笠井亮秀


沿岸生態系に果たす河川の役割 

高知大学研究拠点プロジェクト 革新的な水・バイオマス循環システムの構築 公開シンポジウム,2017年12月2日,ちより街テラス

笠井亮秀


The effect of land use structure in the catchment area on ecosystems in coastal seas

The JSFS 85th Anniversary-Commemorative International Symposium "Fisheries Science for Future Generations",  22-24 September 2017, Tokyo University of Marine Science and Technology

Akihide Kasai



Presentation in scientific meeting

環境 DNA分析によるエスチュアリーにおける魚類の多様性評価

2019年度水産海洋学会,2019年11月8日~10日,東北大学農学部

笠井亮秀・安 孝珍・久米 学・寺島祐樹・Ye Feng・亀山 哲・宮 正樹・山下 洋


環境 DNA手法を用いた日本縁辺部におけるニホンウナギの分布可能性の解明

2019年度水産海洋学会,2019年11月8日~10日,東北大学農学部

定行洋亮・笠井亮秀・山下 洋・東 信行・山中裕樹・亀山 哲・久米 学・和田敏裕・木村伸吾

Seasonal changes of fish biodiversity in Hakodate Bay, Japan, revealed by eDNA metabarcoding technique

The fourth Asian marine biology symposium, 4-6 November 2019, National Taiwan University

Hyojin Ahn, Akihide Kasai and Yoh Yamashita


環境DNA分析によるニホンウナギの分布推定

2019年度環境DNA学会第2回神戸大会,2019年11月4日,神戸大学鶴甲第2キャンパス

笠井亮秀


環境DNAを用いた全国31河川河口域における魚類群集構造の解明

2019年度日本魚類学会年会,2019年9月20~23日,高知大学朝倉キャンパス

久米学・安孝珍・寺島佑樹・Edouard Lavergne・Feng Ye・亀山哲・邉見由美・甲斐嘉晃・伊勢武史・笠井亮秀・山下洋


Evaluation of biodiversity at five estuaries in Japan using environmental DNA  metabarcoding method

54th European marine biology symposium, 25 - 29 August 2019, University College Dublin

Hyojin Ahn, Manabu Kume, Yuki Terashima, Feng Ye, Satoshi Kameyama, Masaki Miya, Yoh Yamashita and Akihide Kasai


福島県松川浦と流入河におけるニホンウナギの摂餌生態

平成31年度日本水産学会春季大会,2019年3月26日~30日,東京海洋大学

Alisa Kutzer Alisa Kutzer・小巻拓平・野田 琢嗣・和田敏裕・久米 学・寺島佑樹・堀 友彌・高木淳一・三田村啓理・笠井亮秀・荒井修亮・山下 洋


環境DNAを用いた全国河川のニホンウナギ分布調査 

平成31年度日本水産学会春季大会,2019年3月26日~30日,東京海洋大学

笠井亮秀・高田真悟・山崎 彩・定行洋亮・木村伸吾・安 孝珍・寺島佑樹・久米 学・益田玲爾・山下 洋・山中裕樹・今藤夏子・亀山哲・東 信行

Comparison and integration of different ecosystem models for appropriate use of model characteristics

4th CLIOTOP symposium, 15-19 October 2018, National Taiwan Ocean University, Keelung, Taiwan

Aigo Takeshige, Shiroh Yonezaki, Sachihiko Itoh, Akihide Kasai, Masashi Kiyota and Shingo Kimura

Quantitative evaluation of ecosystem services supporting the growth of Japanese chum salmon

4th CLIOTOP symposium, 15-19 October, 2018, National Taiwan Ocean University, Keelung, Taiwan

Karasawa Y, Ueno H, Tanisugi R, Yoon S, Kasai A and Kiyota M


日本系サケの成長を通じた生態系サービスの定量的評価

2018年度水産海洋学会研究発表大会,2018年11月16日~18日,東京大学大気海洋研究所

柄沢有香・上野洋路・谷杉諒・尹錫鎭・笠井亮秀・清田雅史

環境DNAによるニホンウナギの分布域の推定

2018年度水産海洋学会研究発表大会,2018年11月16日~18日,東京大学大気海洋研究所

笠井亮秀・定行洋亮・高田真悟・安孝珍・山崎彩・久米学・寺島佑樹・東信行・亀山哲・木村伸吾・山中裕樹 ・益田玲爾・山下洋


水温と塩分がニホンウナギの環境DNA放出量に及ぼす影響

2018年度水産海洋学会研究発表大会,2018年11月16日~18日,東京大学大気海洋研究所

高田真悟・高橋さやか・益田玲爾・笠井亮秀

環境DNA濃度の定量データと流動モデルの統合による個体数・生物量の推定

第1回環境DNA学会東京大会,2018年9月29日~30日,日本科学未来館

深谷肇一・村上弘章・尹錫鎭・南憲吏・長田穣・山本哲史・益田玲爾・笠井亮秀・宮下和士・源利文・近藤倫生


Evaluation of the fish distribution and their environmental DNA in a semi-closed bay

using a three-dimensional tracer model

第1回環境DNA学会東京大会,2018年9月29日~30日,日本科学未来館

Seokjin Yoon・笠井亮秀・村上弘章・南憲吏・益田玲爾・宮下和士・源利文・近藤倫生


Nutrients, chlorophyll and suspended solids from three rivers into Hakodate Bay, Hokkaido, Japan

ECSA 57, 3-6 September 2018, Pan Pacific Perth, Australia

A. Kasai, R. Sugimoto, N. Itoh, K. Tsukagawa, and K. Todate